Estimation of data-specific constitutive exons with RNA-Seq data.

ABSTRACT:

Background

RNA-Seq has the potential to answer many diverse and interesting questions about the inner workings of cells. Estimating changes in the overall transcription of a gene is not straightforward. Changes in overall gene transcription can easily be confounded with changes in exon usage which alter the lengths of transcripts produced by a gene. Measuring the expression of constitutive exons--xons which are consistently conserved after splicing--ffers an unbiased estimation of the overall transcription of a gene.

Results

We propose a clustering-based method, exClust, for estimating the exons that are consistently conserved after splicing in a given data set. These are considered as the exons which are "constitutive" in this data. The method utilises information from both

SUBMITTER: Patrick E 

PROVIDER: S-EPMC3656776 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature