Targeted analysis and discovery of posttranslational modifications in proteins from methanogenic archaea by top-down MS.

ABSTRACT: For more complete characterization of DNA-predicted proteins (including their posttranslational modifications) a "top-down" approach using high-resolution tandem MS is forwarded here by its application to methanogens in both hypothesis-driven and discovery modes, with the latter dependent on new automation benchmarks for intact proteins. With proteins isolated from ribosomes and whole-cell lysates of Methanococcus jannaschii (approximately 1,800 genes) using a 2D protein fractionation method, 72 gene products were identified and characterized with 100% sequence coverage via automated fragmentation of intact protein ions in a custom quadrupole/Fourier transform hybrid mass spectrometer. Three incorrect start sites and two modifications were found, with one of each determined for MJ0556, a 2

SUBMITTER: Forbes AJ 

PROVIDER: S-EPMC365680 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature