The human gut chip "HuGChip", an explorative phylogenetic microarray for determining gut microbiome diversity at family level.

ABSTRACT: Evaluating the composition of the human gut microbiota greatly facilitates studies on its role in human pathophysiology, and is heavily reliant on culture-independent molecular methods. A microarray designated the Human Gut Chip (HuGChip) was developed to analyze and compare human gut microbiota samples. The PhylArray software was used to design specific and sensitive probes. The DNA chip was composed of 4,441 probes (2,442 specific and 1,919 explorative probes) targeting 66 bacterial families. A mock community composed of 16S rRNA gene sequences from intestinal species was used to define the threshold criteria to be used to analyze complex samples. This was then experimentally verified with three human faecal samples and results were compared (i) with pyrosequencing of the V4 hypervariabl

SUBMITTER: Tottey W 

PROVIDER: S-EPMC3656878 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature