Solvation in protein folding analysis: combination of theoretical and experimental approaches.

ABSTRACT: An effort to combine theoretical analyses and protein engineering methods has been made to probe the folding mechanism of SH3 by using Energy Landscape Theory and a phi-value analysis. Particular emphasis was given to core residues and the effect of desolvation during the folding event by replacing the core valines with isosteric threonines. These mutations have the advantage of keeping the core structurally invariant while affecting core stability relative to the unfolded state. Although the valines that form the core appear spatially invariant, the folding kinetics of their threonine mutants varies, indicating their different extent of solvation in the transition-state ensemble. Theoretical studies predicted the distribution of folding kinetics of threonine mutants without previous knowl

SUBMITTER: Fernandez-Escamilla AM 

PROVIDER: S-EPMC365706 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature