Multivariate proteomic analysis of murine embryonic stem cell self-renewal versus differentiation signaling.

ABSTRACT: A number of extracellular stimuli, including soluble cytokines and insoluble matrix factors, are known to influence murine embryonic stem cell self-renewal and differentiation behavioral responses via intracellular signaling pathways, but their net effects in combination are difficult to understand. To gain insight concerning key intracellular signals governing these behavioral responses, we employ a multivariate systems analysis of proteomic data generated from combinatorial stimulation of mouse embryonic stem cells by fibronectin, laminin, leukemia-inhibitory factor, and fibroblast growth factor 4. Phosphorylation states of 31 intracellular signaling network components were obtained across 16 different stimulus conditions at three time points by quantitative Western blotting, and partial

SUBMITTER: Prudhomme W 

PROVIDER: S-EPMC365717 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature