Foetal stem cell derivation & characterization for osteogenic lineage.

ABSTRACT:

Background & objectives

Mesencymal stem cells (MSCs) derived from foetal tissues present a multipotent progenitor cell source for application in tissue engineering and regenerative medicine. The present study was carried out to derive foetal mesenchymal stem cells from ovine source and analyze their differentiation to osteogenic linage to serve as an animal model to predict human applications.

Methods

Isolation and culture of sheep foetal bone marrow cells were done and uniform clonally derived MSC population was collected. The cells were characterized using cytochemical, immunophenotyping, biochemical and molecular analyses. The cells with defined characteristics were differentiated into osteogenic lineages and analysis for differentiated cell types was done. The cells were

SUBMITTER: Gowri AM 

PROVIDER: S-EPMC3657854 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature