Basic and applied uses of genome-scale metabolic network reconstructions of Escherichia coli.

ABSTRACT: The genome-scale model (GEM) of metabolism in the bacterium Escherichia coli K-12 has been in development for over a decade and is now in wide use. GEM-enabled studies of E. coli have been primarily focused on six applications: (1) metabolic engineering, (2) model-driven discovery, (3) prediction of cellular phenotypes, (4) analysis of biological network properties, (5) studies of evolutionary processes, and (6) models of interspecies interactions. In this review, we provide an overview of these applications along with a critical assessment of their successes and limitations, and a perspective on likely future developments in the field. Taken together, the studies performed over the past decade have established a genome-scale mechanistic understanding of genotype-phenotype relationships in

SUBMITTER: McCloskey D 

PROVIDER: S-EPMC3658273 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature