Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12.

ABSTRACT: The expressions of reference genes used in gene expression studies are assumed to be stable under most circumstances. However, a number of studies had demonstrated that such genes were found to vary under experimental conditions. In addition, genes that are stably expressed in an organ may not be stably expressed in other organs or other organisms, suggesting the need to identify reference genes for each organ and organism. This study aims at identifying stably expressed genes in Escherichia coli. Microarray datasets from E. coli substrain MG1655 and 1 dataset from W3110 were analysed. Coefficient of variance (COV) of was calculated and 10% of the lowest COV from 4631 genes common in the 3 MG1655 sets were analysed using NormFinder. Glucan biosynthesis protein G (mdoG), which is involved i

SUBMITTER: Heng SS 

PROVIDER: S-EPMC3658596 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature