Comparative transcriptome analysis and marker development of two closely related Primrose species (Primula poissonii and Primula wilsonii).

ABSTRACT:

Background

Primula species are important early spring garden plants with a centre of diversity and speciation in the East Himalaya-Hengduan Mountains in Western China. Studies on population genetics, speciation and phylogeny of Primula have been impeded by a lack of genomic resources. In the present study, we sequenced the transcriptomes of two closely related primrose species, Primula poissonii and Primula wilsonii, using short reads on the Illumina Genome Analyzer platform.

Results

We obtained 55,284 and 55,011 contigs with N50 values of 938 and 1,085 for P. poissonii and P. wilsonii, respectively, and 6,654 pairs of putative orthologs were identified between the two species. Estimations of non-synonymous/synonymous substitution rate ratios for these orthologs indicated th

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3658987 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature