Variability in the generation of induced pluripotent stem cells: importance for disease modeling.

ABSTRACT: In the field of disease modeling, induced pluripotent stem cells (iPSCs) have become an appealing choice, especially for diseases that do not have an animal model. They can be generated from patients with known clinical features and compared with cells from healthy controls to identify the biological bases of disease. This study was undertaken to determine the variability in iPSC lines derived from different individuals, with the aim of determining criteria for selecting iPSC lines for disease models. We generated and characterized 18 iPSC lines from eight donors and considered variability at three levels: (a) variability in the criteria that define iPSC lines as pluripotent cells, (b) variability in cell lines from different donors, and (c) variability in cell lines from the same donor. W

SUBMITTER: Vitale AM 

PROVIDER: S-EPMC3659735 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature