Six novel Y chromosome genes in Anopheles mosquitoes discovered by independently sequencing males and females.

ABSTRACT:

Background

Y chromosomes are responsible for the initiation of male development, male fertility, and other male-related functions in diverse species. However, Y genes are rarely characterized outside a few model species due to the arduous nature of studying the repeat-rich Y.

Results

The chromosome quotient (CQ) is a novel approach to systematically discover Y chromosome genes. In the CQ method, genomic DNA from males and females is sequenced independently and aligned to candidate reference sequences. The female to male ratio of the number of alignments to a reference sequence, a parameter called the chromosome quotient (CQ), is used to determine whether the sequence is Y-linked. Using the CQ method, we successfully identified known Y sequences from Homo sapiens and Drosophi

SUBMITTER: Hall AB 

PROVIDER: S-EPMC3660176 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature