Human monoclonal ScFv that bind to different functional domains of M2 and inhibit H5N1 influenza virus replication.

ABSTRACT:

Background

Novel effective anti-influenza agent that tolerates influenza virus antigenic variation is needed. Highly conserved influenza virus M2 protein has multiple pivotal functions including ion channel activity for vRNP uncoating, anti-autophagy and virus assembly, morphogenesis and release. Thus, M2 is an attractive target of anti-influenza agents including small molecular drugs and specific antibodies.

Methods

Fully human monoclonal single chain antibodies (HuScFv) specific to recombinant and native M2 proteins of A/H5N1 virus were produced from huscfv-phagemid transformed E. coli clones selected from a HuScFv phage display library using recombinant M2 of clade 1 A/H5N1 as panning antigen. The HuScFv were tested for their ability to inhibit replication of A/H5N1 of bo

SUBMITTER: Pissawong T 

PROVIDER: S-EPMC3660209 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature