Actin mediates the nanoscale membrane organization of the clustered membrane protein influenza hemagglutinin.

ABSTRACT: The influenza viral membrane protein hemagglutinin (HA) is required at high concentrations on virion and host-cell membranes for infectivity. Because the role of actin in membrane organization is not completely understood, we quantified the relationship between HA and host-cell actin at the nanoscale. Results obtained using superresolution fluorescence photoactivation localization microscopy (FPALM) in nonpolarized cells show that HA clusters colocalize with actin-rich membrane regions (ARMRs). Individual molecular trajectories in live cells indicate restricted HA mobility in ARMRs, and actin disruption caused specific changes to HA clustering. Surprisingly, the actin-binding protein cofilin was excluded from some regions within several hundred nanometers of HA clusters, suggesting that HA

SUBMITTER: Gudheti MV 

PROVIDER: S-EPMC3660645 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature