Fas ligand-Fas signaling participates in light-induced apoptotic death in photoreceptor cells.

ABSTRACT:

Purpose

We investigated the function of Fas in photoreceptors.

Methods

Postmortem human eyes and mouse-derived photoreceptor cells (661W) were examined for Fas expression by in situ hybridization and immunofluorescence. 661W cells were treated with FasL or Fas agonistic antibody, or exposed to light with/without pharmacological manipulation of Fas signaling, followed by apoptosis detection by TUNEL, immunofluorescence and fluorescence activated cell scanning (FACS). Fractionated cellular extracts were used to detect protein expression or protein phosphorylation after immunoprecipitation by Western blot.

Results

Fas was expressed in the photoreceptor layer of human retina. Fas and a cleaved form of FasL were found on the cell surface of 661W cells. Treatment with FasL

SUBMITTER: Chang Q 

PROVIDER: S-EPMC3660862 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature