Structure of a class II preQ1 riboswitch reveals ligand recognition by a new fold.

ABSTRACT: PreQ1 riboswitches regulate genes by binding the pyrrolopyrimidine intermediate preQ1 during the biosynthesis of the essential tRNA base queuosine. We report what is to our knowledge the first preQ1-II riboswitch structure at 2.3-Å resolution, which uses a previously uncharacterized fold to achieve effector recognition at the confluence of a three-way helical junction flanking a pseudoknotted ribosome-binding site. The results account for translational control mediated by the preQ1-II riboswitch class and expand the known repertoire of ligand-binding modes used by regulatory RNAs.

SUBMITTER: Liberman JA 

PROVIDER: S-EPMC3661761 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature