Medullospheres from DAOY, UW228 and ONS-76 cells: increased stem cell population and proteomic modifications.

ABSTRACT:

Background

Medulloblastoma (MB) is an aggressive pediatric tumor of the Central Nervous System (CNS) usually treated according to a refined risk stratification. The study of cancer stem cells (CSC) in MB is a promising approach aimed at finding new treatment strategies.

Methodology/principal findings

The CSC compartment was studied in three characterized MB cell lines (DAOY, UW228 and ONS-76) grown in standard adhesion as well as being grown as spheres, which enables expansion of the CSC population. MB cell lines, grown in adherence and as spheres, were subjected to morphologic analysis at the light and electron microscopic level, as well as cytofluorimetric determinations. Medullospheres (MBS) were shown to express increasingly immature features, along with the stem cells m

SUBMITTER: Zanini C 

PROVIDER: S-EPMC3663798 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature