In vitro reconstruction of human junctional and sulcular epithelium.

ABSTRACT:

Background

The aim of this study was to develop and characterize standardized in vitro three-dimensional organotypic models of human junctional epithelium (JE) and sulcular epithelium (SE).

Methods

Organotypic models were constructed by growing human normal gingival keratinocytes on top of collagen matrices populated with gingival fibroblasts (GF) or periodontal ligament fibroblasts (PLF). Tissues obtained were harvested at different time points and assessed for epithelial morphology, proliferation (Ki67), expression of JE-specific markers (ODAM and FDC-SP), cytokeratins (CK), transglutaminase, filaggrin, and basement membrane proteins (collagen IV and laminin1).

Results

The epithelial component in 3- and 5-day organotypics showed limited differentiation and expressed

SUBMITTER: Dabija-Wolter G 

PROVIDER: S-EPMC3664418 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature