Purification and identification of trichloroethylene induced proteins from Stenotrophomonas maltophilia PM102 by immuno-affinity-chromatography and MALDI-TOF Mass spectrometry.

ABSTRACT: A novel bacterial isolate capable of growth on trichloroethylene as the sole carbon source was identified as Stenotrophomonas maltophilia PM102 by 16S rDNA sequencing (GenBank Acc.no. JQ797560). Serum was obtained from a rabbit immunized with the total protein extracted from the PM102 isolate grown in 0.2% TCE with 0.2% peptone. Antibodies to the common antigens were removed by preadsorbing the serum antibody on total protein extracted from the PM102 strain grown in 0.2% peptone. Western blot with the preadsorbed antibody reacted to a single band in TCE and TCE with peptone lane. No reaction was seen in peptone lane. This preadsorbed antibody specific for TCE inducible antigens was immobilised on epoxy activated sepharose 6B and total protein from PM102 cells grown in minimal medium with T

SUBMITTER: Mukherjee P 

PROVIDER: S-EPMC3664754 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature