An engineered small RNA-mediated genetic switch based on a ribozyme expression platform.

ABSTRACT: An important requirement for achieving many goals of synthetic biology is the availability of a large repertoire of reprogrammable genetic switches and appropriate transmitter molecules. In addition to engineering genetic switches, the interconnection of individual switches becomes increasingly important for the construction of more complex genetic networks. In particular, RNA-based switches of gene expression have become a powerful tool to post-transcriptionally program genetic circuits. RNAs used for regulatory purposes have the advantage to transmit, sense, process and execute information. We have recently used the hammerhead ribozyme to control translation initiation in a small molecule-dependent fashion. In addition, riboregulators have been constructed in which a small RNA acts as tr

SUBMITTER: Klauser B 

PROVIDER: S-EPMC3664830 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature