Biglycan induces the expression of osteogenic factors in human aortic valve interstitial cells via Toll-like receptor-2.

ABSTRACT:

Objective

Although biglycan (BGN) and oxidized low-density lipoprotein (oxLDL) accumulation has been observed in calcific, stenotic aortic valves, their role in the pathogenesis of calcific aortic valve disease is poorly understood. We hypothesized that soluble BGN induces the osteogenic response in human aortic valve interstitial cells via Toll-like receptor (TLR) 2 and TLR4 and mediates the proosteogenic effect of oxLDL.

Methods and results

Aortic valve interstitial cells of stenotic valves express higher levels of BGN. Stimulation of cells from normal valves with BGN increased the expression of bone morphogenetic protein-2 (BMP-2) and alkaline phosphatase (ALP) among the chondrogenic/osteogenic markers examined and caused accumulation of calcium deposits. TLR2 silencing,

SUBMITTER: Song R 

PROVIDER: S-EPMC3665416 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature