A genetic screen identifies interferon-α effector genes required to suppress hepatitis C virus replication.

ABSTRACT:

Background & aims

Hepatitis C virus (HCV) infection is a leading cause of end-stage liver disease. Interferon-α (IFNα) is an important component of anti-HCV therapy; it up-regulates transcription of IFN-stimulated genes, many of which have been investigated for their antiviral effects. However, all of the genes required for the antiviral function of IFNα (IFN effector genes [IEGs]) are not known. IEGs include not only IFN-stimulated genes, but other nontranscriptionally induced genes that are required for the antiviral effect of IFNα. In contrast to candidate approaches based on analyses of messenger RNA (mRNA) expression, identification of IEGs requires a broad functional approach.

Methods

We performed an unbiased genome-wide small interfering RNA screen to identify IEGs th

SUBMITTER: Fusco DN 

PROVIDER: S-EPMC3665646 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature