The role of paracrine and autocrine signaling in the early phase of adipogenic differentiation of adipose-derived stem cells.

ABSTRACT:

Introduction

High cell density is known to enhance adipogenic differentiation of mesenchymal stem cells, suggesting secretion of signaling factors or cell-contact-mediated signaling. By employing microfluidic biochip technology, we have been able to separate these two processes and study the secretion pathways.

Methods and results

Adipogenic differentiation of human adipose-derived stem cells (ASCs) cultured in a microfluidic system was investigated under perfusion conditions with an adipogenic medium or an adipogenic medium supplemented with supernatant from differentiating ASCs (conditioned medium). Conditioned medium increased adipogenic differentiation compared to adipogenic medium with respect to accumulation of lipid-filled vacuoles and gene expression of key adipogeni

SUBMITTER: Hemmingsen M 

PROVIDER: S-EPMC3665830 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature