Phenotypic characterization of retinoic acid differentiated SH-SY5Y cells by transcriptional profiling.

ABSTRACT: Multiple genetic and environmental factors play a role in the development and progression of Parkinson's disease (PD). The main neuropathological hallmark of PD is the degeneration of dopaminergic (DAergic) neurons in the substantia nigra pars compacta. To study genetic and molecular contributors to the disease process, there is a great need for readily accessible cells with prominent DAergic features that can be used for reproducible in vitro cellular screening. Here, we investigated the molecular phenotype of retinoic acid (RA) differentiated SH-SY5Y cells using genome wide transcriptional profiling combined with gene ontology, transcription factor and molecular pathway analysis. We demonstrated that RA induces a general neuronal differentiation program in SH-SY5Y cells and that these ce

SUBMITTER: Korecka JA 

PROVIDER: S-EPMC3665836 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature