Dynamic gradients of an intermediate filament-like cytoskeleton are recruited by a polarity landmark during apical growth.

ABSTRACT: Intermediate filament (IF)-like cytoskeleton emerges as a versatile tool for cellular organization in all kingdoms of life, underscoring the importance of mechanistically understanding its diverse manifestations. We showed previously that, in Streptomyces (a bacterium with a mycelial lifestyle similar to that of filamentous fungi, including extreme cell and growth polarity), the IF protein FilP confers rigidity to the hyphae by an unknown mechanism. Here, we provide a possible explanation for the IF-like function of FilP by demonstrating its ability to self-assemble into a cis-interconnected regular network in vitro and its localization into structures consistent with a cytoskeletal network in vivo. Furthermore, we reveal that a spatially restricted interaction between FilP and DivIVA, the

SUBMITTER: Fuchino K 

PROVIDER: S-EPMC3666699 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature