Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites.

ABSTRACT:

Background

Transcription factor overexpression is common in biological experiments and transcription factor amplification is associated with many cancers, yet few studies have directly compared the DNA-binding profiles of endogenous versus overexpressed transcription factors.

Methods

We analyzed MyoD ChIP-seq data from C2C12 mouse myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells by overexpression of MyoD and compared the genome-wide binding profiles and binding site characteristics of endogenous and overexpressed MyoD.

Results

Overexpressed MyoD bound to the same sites occupied by endogenous MyoD and possessed the same E-box sequence preference and co-factor site enrichments, and did not bind to new sites with distinct character

SUBMITTER: Yao Z 

PROVIDER: S-EPMC3666993 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature