Genome-wide distribution of histone H4 Lysine 16 acetylation sites and their relationship to gene expression.

ABSTRACT:

Background

Histone post-translational modifications are critical determinants of chromatin structure and function, impacting multiple biological processes including DNA transcription, replication, and repair. The post-translational acetylation of histone H4 at lysine 16 (H4K16ac) was initially identified in association with dosage compensation of the Drosophila male X chromosome. However, in mammalian cells, H4K16ac is not associated with dosage compensation and the genomic distribution of H4K16ac is not precisely known. Therefore, we have mapped the genome-wide H4K16ac distribution in human cells.

Results

We performed H4K16ac chromatin immunoprecipitation from human embryonic kidney 293 (HEK293) cells followed by hybridization to whole-genome tiling arrays and identified 25

SUBMITTER: Horikoshi N 

PROVIDER: S-EPMC3667149 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature