Molecular profiling of tumor-specific T_H1 cells activated in vivo.

ABSTRACT: The central role of tumor-specific T_H1 cells in anticancer immune responses is becoming increasingly appreciated. However, little is known about how these cells are generated in vivo. Here, we used flow cytometry and gene expression microarrays to characterize the primary activation and T_H1 differentiation of naïve tumor-specific CD4⁺ T cells in a mouse model of cancer immunosurveillance. We took advantage of T-cell receptor-transgenic mice in which CD4⁺ T cells recognize a tumor-specific antigen secreted by MHC class II-negative MOPC315 myeloma cells. Cancer cells were injected subcutaneously and T-cell activation was analyzed in draining lymph nodes and at the incipient tumor site 8 d later. Upon activation and migration to incipient tumor site

SUBMITTER: Lorvik KB 

PROVIDER: S-EPMC3667914 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature