RNA-seq analysis of single bovine blastocysts.

ABSTRACT:

Background

Use of RNA-Seq presents unique benefits in terms of gene expression analysis because of its wide dynamic range and ability to identify functional sequence variants. This technology provides the opportunity to assay the developing embryo, but the paucity of biological material available from individual embryos has made this a challenging prospect.

Results

We report here the first application of RNA-Seq for the analysis of individual blastocyst gene expression, SNP detection, and characterization of allele specific expression (ASE). RNA was extracted from single bovine blastocysts (n = 5), amplified, and analyzed using high-throughput sequencing. Approximately 38 million sequencing reads were generated per embryo and 9,489 known bovine genes were found to be express

SUBMITTER: Chitwood JL 

PROVIDER: S-EPMC3668197 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature