Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii.

ABSTRACT:

Background

The obligate intracellular parasite Toxoplasma gondii can interfere with host cell signaling pathways, alter host defense systems and cell cycle control, and establish a chronic infection in the central nervous system. T. gondii infection may alter the expression profile of host microRNAs (miRNAs) which have key regulatory functions at the post-transcriptional level.

Methods

Using high-throughput sequencing and real-time quantitative PCR technology, we compared the miRNA expression profiles of uninfected mouse brains with brains from mice at 14 days and 21 days after infection with cyst-forming T. gondii (Type II).

Results

A total of 51.30 million raw reads were obtained from all samples and 495 (14d infected mouse sample), 511 (14d sham-infected control),

SUBMITTER: Xu MJ 

PROVIDER: S-EPMC3668261 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature