Identification of novel tissue-specific genes by analysis of microarray databases: a human and mouse model.

ABSTRACT: Understanding the tissue-specific pattern of gene expression is critical in elucidating the molecular mechanisms of tissue development, gene function, and transcriptional regulations of biological processes. Although tissue-specific gene expression information is available in several databases, follow-up strategies to integrate and use these data are limited. The objective of the current study was to identify and evaluate novel tissue-specific genes in human and mouse tissues by performing comparative microarray database analysis and semi-quantitative PCR analysis. We developed a powerful approach to predict tissue-specific genes by analyzing existing microarray data from the NCBI's Gene Expression Omnibus (GEO) public repository. We investigated and confirmed tissue-specific gene expressi

SUBMITTER: Song Y 

PROVIDER: S-EPMC3669334 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature