Modulation of multidrug resistance 1 expression and function in retinoblastoma cells by curcumin.

ABSTRACT:

Objective

To determine the possible interaction of curcumin with P-glycoprotein (P-gp) expression and function by in vitro and in silico studies.

Materials and methods

In this study, curcumin was compared for its potential to modulate the expression and function of P-gp in Y79 RB cells by western blot, RT-PCR (reverse transcription polymerase chain reaction) and functional assay. Further, in silico molecular modeling and docking simulations were performed to deduce the inhibitory binding mode of curcumin.

Results

Western blot and RT-PCR analysis decreased the expression of P-gp in a dose-dependent manner. The effect of curcumin on P-gp function was demonstrated by Rhodamine 123 (Rh123) accumulation and efflux study. Curcumin increased the accumulation of Rh123 and dec

SUBMITTER: Sreenivasan S 

PROVIDER: S-EPMC3669568 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature