Detection of second-line drug resistance in Mycobacterium tuberculosis using oligonucleotide microarrays.

ABSTRACT:

Background

The steady rise in the spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) requires rapid and reliable methods to identify resistant strains. The current molecular methods to detect MTB resistance to second-line drugs either do not cover an extended spectrum of mutations to be identified or are not easily implemented in clinical laboratories. A rapid molecular technique for the detection of resistance to second-line drugs in M. tuberculosis has been developed using hybridisation analysis on microarrays.

Methods

The method allows the identification of mutations within the gyrA and gyrB genes responsible for fluoroquinolones resistance and mutations within the rrs gene and the eis promoter region associated with t

SUBMITTER: Zimenkov DV 

PROVIDER: S-EPMC3671172 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature