Toll-like receptor 4 contributes to poor outcome after intracerebral hemorrhage.

ABSTRACT:

Objective

Intracerebral hemorrhage (ICH) is a devastating stroke subtype in which perihematomal inflammation contributes to neuronal injury and functional disability. Histologically, the region becomes infiltrated with neutrophils and activated microglia followed by neuronal loss, but little is known about the immune signals that coordinate these events. This study aimed to determine the role of Toll-like receptor 4 (TLR4) in the innate immune response after ICH and its impact on neurobehavioral outcome.

Methods

Transgenic mice incapable of TLR4 signaling and wild-type controls were subjected to striatal blood injection to model ICH. The perihematomal inflammatory response was then quantified by immunohistochemistry, whole brain flow cytometry, and polymerase chain reaction.

SUBMITTER: Sansing LH 

PROVIDER: S-EPMC3671585 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature