Expression of cellulosome components and type IV pili within the extracellular proteome of Ruminococcus flavefaciens 007.

ABSTRACT:

Background

Ruminococcus flavefaciens is an important fibre-degrading bacterium found in the mammalian gut. Cellulolytic strains from the bovine rumen have been shown to produce complex cellulosome structures that are associated with the cell surface. R. flavefaciens 007 is a highly cellulolytic strain whose ability to degrade dewaxed cotton, but not Avicel cellulose, was lost following initial isolation in the variant 007S. The ability was recovered after serial subculture to give the cotton-degrading strain 007C. This has allowed us to investigate the factors required for degradation of this particularly recalcitrant form of cellulose.

Methodology/principal findings

The major proteins associated with the bacterial cell surface and with the culture supernatant were analyzed

SUBMITTER: Vodovnik M 

PROVIDER: S-EPMC3672088 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature