Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy.

ABSTRACT:

Objective

To examine gonadal protective properties of granulocyte colony-stimulating factor (G-CSF) alone or in combination with stem cell factor (SCF) in female mice treated with high-dose alkylating chemotherapy.

Design

Experimental laboratory animal study.

Setting

Tertiary care academic hospital and research institute.

Animal(s)

Six- and 8-week-old C57Bl/6 female mice.

Intervention(s)

Adult female mice were treated with [1] cyclophosphamide and busulfan (CTx), [2] CTx + G-CSF/SCF, [3] CTx + G-CSF, or [4] normal saline and dimethyl sulfoxide (DMSO; vehicle control).

Main outcome measure(s)

Follicle counts, microvessel density, cellular response to DNA damage, and litter production.

Result(s)

G-CSF ± SCF increased microvessel density and d

SUBMITTER: Skaznik-Wikiel ME 

PROVIDER: S-EPMC3672312 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature