Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies: potential markers of embryo developmental competence.

ABSTRACT:

Objective

To test the hypothesis that quantification of messenger RNAs originating from the second polar body (PB(2)) provides a noninvasive tool for assessing embryo quality.

Design

Prospective study.

Setting

Hospital-based academic research laboratory.

Animal(s)

CD1 female mice.

Intervention(s)

Metaphase II oocytes obtained from 7- to 8-week-old mice after pregnant mare's serum gonadotropin and hCG priming. After in vitro fertilization, the PB(2) was biopsied from zygote, followed by reverse transcription. Real-time polymerase chain reaction was performed to quantify gene expression levels in single PB(2). The sibling zygotes were continuously cultured to blastocyst stage.

Main outcome measure(s)

Embryo developmental competence and six maternal-

SUBMITTER: Jiao ZX 

PROVIDER: S-EPMC3672332 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature