Quantification of glycopeptides by multiple reaction monitoring liquid chromatography/tandem mass spectrometry.

ABSTRACT: Protein glycosylation has a major influence on functions of proteins. Studies have shown that aberrations in glycosylation are indicative of disease conditions. This has prompted major research activities for comparative studies of glycoproteins in biological samples. Multiple reaction monitoring (MRM) is a highly sensitive technique which has been recently explored for quantitative proteomics. In this work, MRM was adopted for quantification of glycopeptides derived from both model glycoproteins and depleted human blood serum using glycan oxonium ions as transitions. The utilization of oxonium ions aids in identifying the different types of glycans bound to peptide backbones. MRM experiments were optimized by evaluating different parameters that have a major influence on quantification of

SUBMITTER: Song E 

PROVIDER: S-EPMC3673029 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature