Promoter-proximal CCCTC-factor binding is associated with an increase in the transcriptional pausing index.

ABSTRACT:

Motivation

It has been known for more than 2 decades that after RNA polymerase II (RNAPII) initiates transcription, it can enter into a paused or stalled state immediately downstream of the transcription start site before productive elongation. Recent advances in high-throughput genomic technologies facilitated the discovery that RNAPII pausing at promoters is a widespread physiologically regulated phenomenon. The molecular underpinnings of pausing are incompletely understood. The CCCTC-factor (CTCF) is a ubiquitous nuclear factor that has diverse regulatory functions, including a recently discovered role in promoting RNAPII pausing at splice sites.

Results

In this study, we analyzed CTCF binding sites and nascent transcriptomic data from three different cell types, and foun

SUBMITTER: Paredes SH 

PROVIDER: S-EPMC3673211 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature