Dataset Information
Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer.
Ontology highlight
ABSTRACT: Background
Mediator complex subunit 19 (Med19) is a critical subunit of the mediator complex that forms a bridge between the transcription factors and RNA polymerase II. Although it has been reported that Med19 plays an important role in stabilizing the whole mediator complex, its biological importance in tongue cancer cell proliferation and migration has not been addressed.Methods
By using MTT, BrdU incorporation, colony formation, flow cytometric, tumorigenesis and transwell assays, We tested the Med19 role on tongue cancer cell growth and migration.Results
We demonstrated that lentivirus-mediated Med19 knockdown could arrest tongue cancer cells at G1 phase, inhibit tongue cancer cell proliferation and migration in vitro. The tumorigenicity of Med19 short hairpin RNA (shRNA)-expressing lentivirus infected tongue cancer cells were decreased after inoculating into nude mice.Conclusions
These results indicate that Med19 plays an important role in tongue cancer proliferation and migration, and suggest possible applications for tongue cancer therapy.
SUBMITTER: Zhu LJ
PROVIDER: S-EPMC3673833 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer.
World journal of surgical oncology 20130527
<h4>Background</h4>Mediator complex subunit 19 (Med19) is a critical subunit of the mediator complex that forms a bridge between the transcription factors and RNA polymerase II. Although it has been reported that Med19 plays an important role in stabilizing the whole mediator complex, its biological importance in tongue cancer cell proliferation and migration has not been addressed.<h4>Methods</h4>By using MTT, BrdU incorporation, colony formation, flow cytometric, tumorigenesis and transwell as ...[more]