The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.

ABSTRACT: Accurate mitotic spindle positioning is essential for the regulation of cell fate choices, cell size and cell position within tissues. The most prominent model of spindle positioning involves a cortical pulling mechanism, where the minus end-directed microtubule motor protein dynein is attached to the cell cortex and exerts pulling forces on the plus ends of astral microtubules that reach the cortex. In nonpolarized cultured cells integrin-dependent, retraction fiber-mediated cell adhesion is involved in spindle orientation. Proteins serving as intermediaries between cortical actin or retraction fibers and astral microtubules remain largely unknown. In a recent genome-wide RNAi screen we identified a previously uncharacterized protein, MISP (C19ORF21) as being involved in centrosome cluste

SUBMITTER: Maier B 

PROVIDER: S-EPMC3674073 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature