Silencing of microRNA-101 prevents IL-1β-induced extracellular matrix degradation in chondrocytes.

ABSTRACT:

Introduction

Extracellular matrix (ECM) degradation leads to malfunction of the cartilage in osteoarthritis (OA). Inflammatory cytokine interleukin-1 beta (IL-1β) functions in ECM degradation and prevents ECM synthesis by down-regulating the key transcription factor, Sox9, and consequently inhibiting ECM gene expression. Evidence reveals that microRNAs (miRNA) have been associated with OA, but little is known of their function in chondrocyte ECM degradation. This study aimed to identify possible miRNAs that mediate IL-1β-induced down-regulation of Sox9 as well as its known down-stream genes, collagen type II and aggrecan.

Methods

The miRNAs were predicted based on three classical databases. The expression levels of the predicted miRNAs were assessed in IL-1β stimulated chond

SUBMITTER: Dai L 

PROVIDER: S-EPMC3674628 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature