Molecular identification and analysis of human enteroviruses isolated from healthy children in Shenzhen, China from 2010 to 2011.

ABSTRACT:

Objective

To determine the prevalence and distribution of human enteroviruses (HEVs) among healthy children in Shenzhen, China.

Method

Clinical specimens were obtained from 320 healthy children under 5 years old in Shenzhen, China from 2010 to 2011. The specimens were evaluated using real-time PCR and cell cultures. The positive specimens were further tested using reverse transcription-seminested PCR (RT-snPCR). Molecular typing and phylogenetic analysis were based on the sequence determined.

Results

Among the 320 samples, 34 were tested positive for HEVs (10.6%) and 22 different serotypes were identified using RT-snPCR. PV1 and PV2 were also detected. The predominant serotype observed was EV71 (17.6%), followed by CV-B4 (14.7%). HEV-B was detected most frequently, wi

SUBMITTER: Wu W 

PROVIDER: S-EPMC3675095 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature