High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases.

ABSTRACT: Transcription activator-like effector nucleases (TALENs) are a powerful new approach for targeted gene disruption in various animal models, but little is known about their activities in Mus musculus, the widely used mammalian model organism. Here, we report that direct injection of in vitro transcribed messenger RNA of TALEN pairs into mouse zygotes induced somatic mutations, which were stably passed to the next generation through germ-line transmission. With one TALEN pair constructed for each of 10 target genes, mutant F0 mice for each gene were obtained with the mutation rate ranged from 13 to 67% and an average of ∼40% of total healthy newborns with no significant differences between C57BL/6 and FVB/N genetic background. One TALEN pair with single mismatch to their intended target sequ

SUBMITTER: Qiu Z 

PROVIDER: S-EPMC3675477 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature