Quantitation of the dynamic profiles of the innate immune response using multiplex selected reaction monitoring-mass spectrometry.

ABSTRACT: The innate immune response (IIR) is a coordinated intracellular signaling network activated by the presence of pathogen-associated molecular patterns that limits pathogen spread and induces adaptive immunity. Although the precise temporal activation of the various arms of the IIR is a critical factor in the outcome of a disease, currently there are no quantitative multiplex methods for its measurement. In this study, we investigate the temporal activation pattern of the IIR in response to intracellular double-stranded RNA stimulation using a quantitative 10-plex stable isotope dilution-selected reaction monitoring-MS assay. We were able to observe rapid activation of both NF-κB and IRF3 signaling arms, with IRF3 demonstrating a transient response, whereas NF-κB underwent a delayed secondar

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC3675810 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature