Chronic nicotine treatment impacts the regulation of opioid and non-opioid peptides in the rat dorsal striatum.

ABSTRACT: The chronic use of nicotine, the main psychoactive ingredient of tobacco smoking, alters diverse physiological processes and consequently generates physical dependence. To understand the impact of chronic nicotine on neuropeptides, which are potential molecules associated with dependence, we conducted qualitative and quantitative neuropeptidomics on the rat dorsal striatum, an important brain region implicated in the preoccupation/craving phase of drug dependence. We used extensive LC-FT-MS/MS analyses for neuropeptide identification and LC-FT-MS in conjunction with stable isotope addition for relative quantification. The treatment with chronic nicotine for 3 months led to moderate changes in the levels of endogenous dorsal striatum peptides. Five enkephalin opioid peptides were up-regulat

SUBMITTER: Petruzziello F 

PROVIDER: S-EPMC3675813 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature