Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.

ABSTRACT: Identifying the building blocks of mammalian tissues is a precondition for understanding their function. In particular, global and quantitative analysis of the proteome of mammalian tissues would point to tissue-specific mechanisms and place the function of each protein in a whole-organism perspective. We performed proteomic analyses of 28 mouse tissues using high-resolution mass spectrometry and used a mix of mouse tissues labeled via stable isotope labeling with amino acids in cell culture as a "spike-in" internal standard for accurate protein quantification across these tissues. We identified a total of 7,349 proteins and quantified 6,974 of them. Bioinformatic data analysis showed that physiologically related tissues clustered together and that highly expressed proteins represented the

SUBMITTER: Geiger T 

PROVIDER: S-EPMC3675825 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature