Biochemical diversity of carboxyl esterases and lipases from Lake Arreo (Spain): a metagenomic approach.

ABSTRACT: The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46'N, 2°59'W; altitude, 655 m). Together with oligonucleotide usage patterns and BLASTP analysis, our study of esterases/lipases mined from Lake Arreo suggests that its sediment contains moderately halophilic and cold-adapted proteobacteria containing DNA fragments of distantly related plasmids or chromosomal genomic islands of plasmid and phage origins. This metagenome encodes esterases/lipases with broad substrate profiles (tested over a set of 101 structurally diverse esters)

SUBMITTER: Martinez-Martinez M 

PROVIDER: S-EPMC3675924 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature