CENP-A confers a reduction in height on octameric nucleosomes.

ABSTRACT: Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

SUBMITTER: Miell MD 

PROVIDER: S-EPMC3676451 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature