Unknown

Dataset Information

0

QuaNCAT: quantitating proteome dynamics in primary cells.

ABSTRACT: Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

SUBMITTER: Howden AJ 

PROVIDER: S-EPMC3676679 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activat  ...[more]

Similar Datasets

Unknown Proteome-wide acetylation dynamics in human cells.
| S-EPMC5579049 | biostudies-literature
Unknown A proteome map of primary cultured rat Schwann cells.
| S-EPMC3338394 | biostudies-literature
Unknown Proteome signatures of inflammatory activated primary human peripheral blood mononuclear cells.
| S-EPMC3509337 | biostudies-literature
Unknown Quantitating cell-cell interaction functions with applications to glioblastoma multiforme cancer cells.
| S-EPMC3680341 | biostudies-literature
Unknown Quantitating drug-target engagement in single cells in vitro and in vivo.
| S-EPMC5630128 | biostudies-literature
Unknown Quantitating the concentration of Py-Im polyamide-fluorescein conjugates in live cells.
| S-EPMC2587135 | biostudies-literature
Unknown The RootScope: a simple high-throughput screening system for quantitating gene expression dynamics in plant roots.
| S-EPMC3852858 | biostudies-other
Unknown Analysis of human immunodeficiency virus cytopathicity by using a new method for quantitating viral dynamics in cell culture.
| S-EPMC1061548 | biostudies-literature
Unknown Pulsatile dynamics in the yeast proteome.
| S-EPMC4203654 | biostudies-literature
Unknown Proteome profiling of heat shock of human primary breast epithelial cells, a dataset report.
| S-EPMC3118829 | biostudies-literature