QuaNCAT: quantitating proteome dynamics in primary cells.

ABSTRACT: Here we demonstrate quantitation of stimuli-induced proteome dynamics in primary cells by combining the power of bio-orthogonal noncanonical amino acid tagging (BONCAT) and stable-isotope labeling of amino acids in cell culture (SILAC). In conjunction with nanoscale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS), quantitative noncanonical amino acid tagging (QuaNCAT) allowed us to monitor the early expression changes of >600 proteins in primary resting T cells subjected to activation stimuli.

SUBMITTER: Howden AJ 

PROVIDER: S-EPMC3676679 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature