Genome-wide maps of polyadenylation reveal dynamic mRNA 3'-end formation in mammalian cell lineages.

ABSTRACT: Post-transcriptional regulation, often mediated by miRNAs and RNA-binding proteins at the 3' untranslated regions (UTRs) of mRNAs, is implicated in important roles in the output of transcriptome. To decipher this layer of gene regulation, it is essential to measure global mRNA expression quantitatively in a 3'-UTR-specific manner. Here we establish an experimental and bioinformatics pipeline that simultaneously determines 3'-end formation by leveraging local nucleotide composition and quantitatively measures mRNA expression by sequencing polyadenylated transcripts. When applied to purified mouse embryonic skin stem cells and their daughter lineages, we identify 18,060 3' UTRs representing 12,739 distinct mRNAs that are abundantly expressed in the skin. We determine that ∼78% of UTRs are fo

SUBMITTER: Wang L 

PROVIDER: S-EPMC3677251 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature